Significant decrease in the dorsal horn CGRP immunoreactivity caudal to the injury epicenter in SCI-HSSC-treated versus SCI-control animals. CGRP- (A), GAP-43- (B), and Iba1- (C) immunoreactivity in the dorsal horns (DH) caudal of the injury epicenter at two months after L3 SCI. The region of interest (ROI) was defined as outlined in B and C (left panels, red dotted line). A: The quantitative densitometry analysis of CGRP-immunostained images in the dorsal horns of SCI-HSSC-treated animals (A2) showed significantly decreased CGRP expression when compared to SCI-control animals (A1). B, C: The dorsal horn GAP-43 or Iba1 immunoreactivity was not significantly different between experimental groups. (A - C: data expressed as mean ± SEM; student t-tests). (Scale Bars: A - C: 500 μm). CRGP, calcitonin gene-related peptide; GAP-13, growth associated protein 43; HSSC, human fetal spinal cord-derived neural stem cells; Iba1, ionized calcium binding adaptor molecule 1; SCI, spinal cord injury.