Variable | Group 1 | Group 2 | Group 3 | Group 4 | Group 5 | P value |
---|
Caspase 3 | 1* | 1.24 ± 0.15† | 0.91 ± 0.09‡ | 0.88 ± 0.07‡ | 0.6 ± 0.06§ | <0.0001 |
Bcl-2 | 1* | 0.60 ± 0.06† | 0.73 ± 0.06‡ | 0.77 ± 0.06‡ | 0.94 ± 0.09§ | <0.0009 |
TNF-α | 1* | 1.21 ± 0.09† | 0.88 ± 0.07‡ | 0.84 ± 0.04‡ | 0.56 ± 0.07§ | <0.0001 |
MMP-9 | 1* | 1.32 ± 0.1† | 0.94 ± 0.08‡ | 0.92 ± 0.04‡ | 0.69 ±0.05§ | <0.0001 |
RANTES | 1* | 1.30 ± 0.04† | 1.03 ± 0.04‡ | 1.08 ± 0.06‡ | 0.84 ± 0.07§ | <0.003 |
NOX-1 | 1* | 1.30 ± 0.09† | 1.07 ± 0.05‡ | 1.03 ± 0.04‡ | 0.89 ± 0.05§ | <0.007 |
HO-1 | 1* | 1.43 ± 0.10† | 1.66 ± 0.09‡ | 1.92 ± 0.11§ | 2.32 ± 0.11¶ | <0.0001 |
NQO 1 | 1* | 1.1 ± 0.07† | 1.22 ± 0.09‡ | 1.32 ± 0.04§ | 1.44 ± 0.10¶ | <0.0001 |
IL-10 | 1* | 1.66 ± 0.07† | 1.60 ± 0.11‡ | 2.09 ± 0.06§ | 2.75 ± 0.11¶ | <0.0001 |
PGE-2 | 1* | 0.82 ± 0.10† | 1.05 ± 0.07‡ | 1.17 ± 0.05§ | 1.29 ± 0.07¶ | <0.0001 |
eNOS | 1* | 0.62 ± 0.04† | 0.75 ± 0.05‡ | 0.89 ± 0.05§ | 1.11 ± 0.04* | <0.0001 |
- Relative changes in mRNA expression of apoptotic, inflammatory, oxidative, antioxidant, and anti-inflammatory mediators in renal parenchyma after ischemia–reperfusion injury (n = 8 in each group). Data expressed as mean ± standard deviation. eNOS, endothelial nitric oxide synthase; HO-1, heme oxygenase 1; MMP, matrix metalloproteinase; NOX, NAD(P)H oxidase; NQO, NAD(P)H quinone oxidoreductase; PGE2, prostaglandin E2; RANTES, regulated and normal T-cell expressed and presumably secreted. Group 1 = sham control; group 2 = ischemia–reperfusion (IR); group 3 = IR + cyclosporine (CsA); group 4 = IR + adipose-derived mesenchymal stem cells (ADMSC); group 5 = IR + CsA + ADMSC. Statistical analysis in each group by analysis of variance followed by Bonferroni multiple comparison post-hoc test. Symbols (*, †, ‡, §, ¶) indicate significance (at 0.05 level) by Bonferroni multiple-comparison post-hoc test: † vs. ‡ vs. § vs. ¶, all P <0.05 for inter-group comparisons.