|
Variable
|
Group 1
|
Group 2
|
Group 3
|
Group 4
|
Group 5
|
P value
|
|---|
|
Caspase 3
|
1*
|
1.24 ± 0.15†
|
0.91 ± 0.09‡
|
0.88 ± 0.07‡
|
0.6 ± 0.06§
|
<0.0001
|
|
Bcl-2
|
1*
|
0.60 ± 0.06†
|
0.73 ± 0.06‡
|
0.77 ± 0.06‡
|
0.94 ± 0.09§
|
<0.0009
|
|
TNF-α
|
1*
|
1.21 ± 0.09†
|
0.88 ± 0.07‡
|
0.84 ± 0.04‡
|
0.56 ± 0.07§
|
<0.0001
|
|
MMP-9
|
1*
|
1.32 ± 0.1†
|
0.94 ± 0.08‡
|
0.92 ± 0.04‡
|
0.69 ±0.05§
|
<0.0001
|
|
RANTES
|
1*
|
1.30 ± 0.04†
|
1.03 ± 0.04‡
|
1.08 ± 0.06‡
|
0.84 ± 0.07§
|
<0.003
|
|
NOX-1
|
1*
|
1.30 ± 0.09†
|
1.07 ± 0.05‡
|
1.03 ± 0.04‡
|
0.89 ± 0.05§
|
<0.007
|
|
HO-1
|
1*
|
1.43 ± 0.10†
|
1.66 ± 0.09‡
|
1.92 ± 0.11§
|
2.32 ± 0.11¶
|
<0.0001
|
|
NQO 1
|
1*
|
1.1 ± 0.07†
|
1.22 ± 0.09‡
|
1.32 ± 0.04§
|
1.44 ± 0.10¶
|
<0.0001
|
|
IL-10
|
1*
|
1.66 ± 0.07†
|
1.60 ± 0.11‡
|
2.09 ± 0.06§
|
2.75 ± 0.11¶
|
<0.0001
|
|
PGE-2
|
1*
|
0.82 ± 0.10†
|
1.05 ± 0.07‡
|
1.17 ± 0.05§
|
1.29 ± 0.07¶
|
<0.0001
|
|
eNOS
|
1*
|
0.62 ± 0.04†
|
0.75 ± 0.05‡
|
0.89 ± 0.05§
|
1.11 ± 0.04*
|
<0.0001
|
- Relative changes in mRNA expression of apoptotic, inflammatory, oxidative, antioxidant, and anti-inflammatory mediators in renal parenchyma after ischemia–reperfusion injury (n = 8 in each group). Data expressed as mean ± standard deviation. eNOS, endothelial nitric oxide synthase; HO-1, heme oxygenase 1; MMP, matrix metalloproteinase; NOX, NAD(P)H oxidase; NQO, NAD(P)H quinone oxidoreductase; PGE2, prostaglandin E2; RANTES, regulated and normal T-cell expressed and presumably secreted. Group 1 = sham control; group 2 = ischemia–reperfusion (IR); group 3 = IR + cyclosporine (CsA); group 4 = IR + adipose-derived mesenchymal stem cells (ADMSC); group 5 = IR + CsA + ADMSC. Statistical analysis in each group by analysis of variance followed by Bonferroni multiple comparison post-hoc test. Symbols (*, †, ‡, §, ¶) indicate significance (at 0.05 level) by Bonferroni multiple-comparison post-hoc test: † vs. ‡ vs. § vs. ¶, all P <0.05 for inter-group comparisons.
