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Table 2 mRNA expression of apoptotic, inflammatory, oxidative, antioxidant, and anti-inflammatory mediators in renal parenchyma after injury

From: Cyclosporine-assisted adipose-derived mesenchymal stem cell therapy to mitigate acute kidney ischemia–reperfusion injury

Variable

Group 1

Group 2

Group 3

Group 4

Group 5

P value

Caspase 3

1*

1.24 ± 0.15

0.91 ± 0.09

0.88 ± 0.07

0.6 ± 0.06§

<0.0001

Bcl-2

1*

0.60 ± 0.06

0.73 ± 0.06

0.77 ± 0.06

0.94 ± 0.09§

<0.0009

TNF-α

1*

1.21 ± 0.09

0.88 ± 0.07

0.84 ± 0.04

0.56 ± 0.07§

<0.0001

MMP-9

1*

1.32 ± 0.1

0.94 ± 0.08

0.92 ± 0.04

0.69 ±0.05§

<0.0001

RANTES

1*

1.30 ± 0.04

1.03 ± 0.04

1.08 ± 0.06

0.84 ± 0.07§

<0.003

NOX-1

1*

1.30 ± 0.09

1.07 ± 0.05

1.03 ± 0.04

0.89 ± 0.05§

<0.007

HO-1

1*

1.43 ± 0.10

1.66 ± 0.09

1.92 ± 0.11§

2.32 ± 0.11

<0.0001

NQO 1

1*

1.1 ± 0.07

1.22 ± 0.09

1.32 ± 0.04§

1.44 ± 0.10

<0.0001

IL-10

1*

1.66 ± 0.07

1.60 ± 0.11

2.09 ± 0.06§

2.75 ± 0.11

<0.0001

PGE-2

1*

0.82 ± 0.10

1.05 ± 0.07

1.17 ± 0.05§

1.29 ± 0.07

<0.0001

eNOS

1*

0.62 ± 0.04

0.75 ± 0.05

0.89 ± 0.05§

1.11 ± 0.04*

<0.0001

  1. Relative changes in mRNA expression of apoptotic, inflammatory, oxidative, antioxidant, and anti-inflammatory mediators in renal parenchyma after ischemia–reperfusion injury (n = 8 in each group). Data expressed as mean ± standard deviation. eNOS, endothelial nitric oxide synthase; HO-1, heme oxygenase 1; MMP, matrix metalloproteinase; NOX, NAD(P)H oxidase; NQO, NAD(P)H quinone oxidoreductase; PGE2, prostaglandin E2; RANTES, regulated and normal T-cell expressed and presumably secreted. Group 1 = sham control; group 2 = ischemia–reperfusion (IR); group 3 = IR + cyclosporine (CsA); group 4 = IR + adipose-derived mesenchymal stem cells (ADMSC); group 5 = IR + CsA + ADMSC. Statistical analysis in each group by analysis of variance followed by Bonferroni multiple comparison post-hoc test. Symbols (*, †, ‡, §, ¶) indicate significance (at 0.05 level) by Bonferroni multiple-comparison post-hoc test: † vs. ‡ vs. § vs. ¶, all P <0.05 for inter-group comparisons.