Figure 9
Rat hepatocyte growth factor (HGF) synthesis in the injured rat TECs after 24 hours or 48 hours of incubation with CM or not. (A) Release of rat HGF by rat TECs. A total absence of rat HGF appeared in the medium conditioned by WJ-MSCs, SFM, or EpiCM. After 24 hours or 48 hours of incubation, CM strongly amplified the release of rat HGF by rat TECs subjected to hypoxia injury when compared with SFM. The ELISA results were normalized by cell numbers. Data are expressed as mean ± SD of three experiments. ∗P = 0.003, CM+EpiCM+TECs versus SFM+EpiCM+TECs; #P < 0.05, CM+EpiCM+TECs versus SFM+EpiCM+TECs; CM, conditioned medium from WJ-MSCs; SFM, serum-free medium; EpiCM, epithelial cell medium. (B) Rat HGF gene expression in rat TECs. Rat HGF gene expression in hypoxia-injured rat TECs was enhanced by CM from WJ-MSCs at 24 hours or 48 hours of incubation. Statistical significance was not achieved at 48 hours. Rat HGF mRNA was undetermined in WJ-MSCs, as a negative control. The Ct (threshold cycle) for rat HGF and β-actin was determined for each sample. The quantification of HGF was normalized by β-actin. HGF expression in TECs incubated without CM was regarded as the calibrator (dotted line).The relative expression of the target gene was calculated by 2-ΔΔCt. Data are expressed as mean of 2-ΔΔCt ± SD of three experiments. ∗P < 0.05, CM+EpiCM+TECs versus SFM+EpiCM+TECs.