Figure 5

IL-10 production from supernatants of coculture of Th1 and Th17 cells with MSCs and functional assay. (A) IL-10 production was quantified in the supernatants of Th1 and Th17 cells co-cultured in the presence or absence of MSCs. * = P <0.05, comparing to Th1 and Th17 cells as control group. All the values represent means ± SED of three independent experiments. B) Functional effect of iTreg cells generated using Th1 and Th17 cells in cocultures with MSCs (conditioned Th1 or Th17, Th1^cond or Th17^cond, respectively). The suppressive capacity of Th1^cond or Th17^cond was assessed by following the number of cell divisions of ConA-stimulated T cells, which were labeled by CFSE. Different ratios of Th^cond:CD4⁺ (1:1; 1:2 or 1:5) were tested. ** = P <0.01 and * = P <0.05. All the values represent means ± SED of three independent experiments for Th1 and Th17 cells. Th1^cond and Th17 ^cond = Th1 and Th17 cells that were co-cultured with MSCs added at different time points of the differentiation processes. CFSE, carboxyfluorescein succinimidyl ester; ConA, concanavalin A; iTreg, induced regulatory T cells; MSCs, mesenchymal stem cells; Th, T helper.