Figure 6From: Conditionally immortalized stem cell lines from human spinal cord retain regional identity and generate functional V2a interneurons and motorneuronsDifferentiated SPC-01 displays spontaneous calcium oscillations. SPC-01 cells were analyzed in the NL buffer for spontaneous oscillations in [Ca2+]i in the presence (Trace A: three examples) or absence of 2 mM external Ca2+ (Trace B). Note that the Ca2+ oscillations were abolished in the absence of external Ca2+.Back to article page