Figure 1

Cell culture and induction of NSCs from iPSCs (bar = 50 μm). The stages for neuronal differentiation (A). iPSCs culture and identification (B through H). iPSCs maintained on a feeder layer tended to form packed clones with a high nucleus-to-cytoplasm ratio (B), displayed a high level of alkaline phosphatase (C), RT-PCR shows that iPSCs expressed pluripotent genes Oct4, Sox2, Nanog (D). EBs in suspending condition for 4 days and initiated the differentiation program (E), spheres formed neural-tube like rosette structures (F), neural-tube-like rosette structures in the center of adherent spheres (G), network-like structure formed after the spheres were cultured in a laminin-coated flask for 1 month (H).