Figure 2

Renal function and structure alteration after MRPC, MRPC/EPO, and MRPC/suramin injection. (A) Gross morphologies of hemisected kidneys in normal or PBS- (postive control), MRPC-, MRPC/EPO-, or MRPC/suramin- treated C57BL/6 mice at one, two, and three days after ischemia-reperfusion injury. (B-N) Representative photomicrographs of H &E stained kidney sections before induction (B), and after two days (C-F), four days (G-J), and seven days (K-N) of acute renal failure in mice treated with PBS (postive control), MRPC, MRPC/EPO, or MRPC/suramin. Acute tubular necrosis induced by I/R injury involving mainly proximal tubules is seen in light micrographs. Luminal debris, loss of brush borders are observed in all groups (black arrow) (Magnification 200×). (O) Jablonski grading scale of histological appearance of acute tubular necrosis from mice subjected to renal ischemia treated with PBS (PC), MRPC, MRPC/EPO or with MRPC/suramin. (P) Serial BUN and Cr levels of acute ischemic injured mice (n = 6 in each group) in postive control, MRPC, MRPC/EPO and MRPC/suramin groups (*, P <0.05; **, P <0.01). BUN, blood urea nitrogen; Cr, serum creatinine; EPO, erythropoietin; I/R, ischemia/reperfusion; MRPC, mouse renal progenitor cells.