Mesenchymal differentiation potential of subpopulations of PLCSCs. PLCSCs were sorted into subpopulations (A, CD34+CD105+; B, CD34-CD105+; C, CD34-CD105-) and put through adipogenic, chondrogenic, and osteogenic differentiation protocols and comparative qPCR analysis (a through i) were performed. Gene expression for adipogenesis (a, FASN; d, PLIN; g PPARG; chondrogenesis (b, ACAN; e, COMP; h, SOX9); osteogenesis (c, BMP4; f, BMP6; I, OPG). Values are represented as mean ± SD, and experiments were repeated in triplicate, each with n = 3. P values for gene expression are indicated, and statistically significant values are represented (*P < 0.01; **P < 0.001).