Figure 7
hBM-MSCs and minocycline in combination reduce apoptosis in EAE mouse spinal cord. (A) Apoptotic cell death was examined with TUNEL staining. TUNEL-positive cells are red, and counterstaining for DAPI is blue. Scale bar, 200 μm. (B) TUNEL-positive cells were also quantified with MetaMorph image analysis. A statistically significant reduction in the number of apoptotic cells in the hBM-MSCs or minocycline treatment-alone group compared with the PBS-treated group (P < 0.001), and the combination treatment showed a more significant decrease in apoptosis compared to hBM-MSCs or minocycline treatment alone (P < 0.001). Columns, mean; bars, SEM. **P < 0.01, ***P < 0.001, one-way ANOVA with post hoc Bonferroni corrections. The results are representative of three independent experiments.