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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Isolation method and xeno-free culture conditions influence multipotent differentiation capacity of human Wharton’s jelly-derived mesenchymal stem cells

Figure 2

Assessment of morphology and proliferation rate of MSCs isolated and subcultured in XF medium. A. Morphological evaluation of primary MSC cultures derived from WJ explants (explant-MSCs -1a, 1b) and enzymatically dissociated WJ (pellet-MSCs -2a, 2b); B. Morphological evaluation of P5 subcultures of WJ explant (3a, 3b)- and pellet (4a, 4b)-derived MSCs; higher magnifications of corresponding pictures are pointed by arrows; C. MTT assay on WJ explant (1)- and pellet (2)-derived MSCs at P2; d, day of assessment; *, **, ***, P < 0.05, indicates statistical significance in cell proliferation during consecutive time points of assessment (*, Day 3 vs. Day 1; **, Day 5 vs. Day 3; *** days 7, 9, 13 vs. Day 5). MSCs, mesenchymal stem cells; P, passage; WJ, Wharton’s jelly; XF, xeno-free.

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Stem Cell Research & Therapy

ISSN: 1757-6512