Figure 5
CCL25-mediated chemotaxis for undifferentiated, chondrogenic differentiated, and dedifferentiated states of MSCs. (A) In CCL25-mediated chemotaxis, the cell-migration assay showed almost uniform hemacolor staining for undifferentiated (D), chondrogenic differentiated (G), and dedifferentiated states of MSCs compared with their (B, E, and H) respective controls. (F) Quantification assessment with Image J software confirmed a relatively low level of migration for chondrogenically differentiated cells (12%) compared with (C) undifferentiated and (I) dedifferentiated states of MSCs. (J) The percentage migration analysis relative to total cell number (30 × 103) is given for undifferentiated, chondrogenic differentiated, and dedifferentiated states of MSCs (n = 3). Student t test was performed for statistical analysis, and asterisks were assigned in the order P* < 0.05, P** < 0.01, and P*** < 0.001, mean ± SEM. Bar, 200 μ m.