Table 2 Commercially available serum-free media for expansion of multipotential stromal cells.
From: Growth factor regulation of proliferation and survival of multipotential stromal cells
| Â | Serum-free media | Company | Properties | Drawbacks |
|---|---|---|---|---|
1 | STEMPRO([R])MSC SFM | Invitrogen | Serum-free, xeno-free. Maintains MSCs for up to nine passages as compared with five passages with MSCs in MEM + 10% FBS. Cells are smaller in size [88] | Marketed as a research product only. Proprietary composition makes it difficult to be used for preclinical and clinical purposes |
2 | Mesencult | Stem Cell Technologies | Serum free, xeno-free. Causes rapid expansion of cells in the first passage, higher than any other media(89) | MSCs fail to maintain a similar growth rate beyond the first passage and stop growing altogether after the sixth passage, while MSCs grown in DMEM-KO and DMEM F12 supplanted with 10% FBS proliferate for up to 25 passages [89] |
3 | Mesengro | StemRD | Chemically defined, serum free and xeno-free. The company claims that the growth rate of MSCs in this media is the same as that of MSCs supplanted with 10% FBS for up to nine passages in vitro | No published data using this media as yet |