Skip to main content

Advertisement

Table 3 ∆C t values for target genes (target C t – β2-microglobulin C t )

From: Human umbilical cord mesenchymal stromal cells exhibit immature nucleus pulposus cell phenotype in a laminin-rich pseudo-three-dimensional culture system

Sample Aggrecan Collagen II ITGα6 ITGα3 ITGβ4 CD239
Control       
Day 1 21.1 ± 0.7 17.6 ± 0.2 14.6 ± 1.0 8.7 ± 1.0 11.2 ± 0.5 13.3 ± 0.4
 Day 7 23.4 ± 0.3 18.3 ± 0.3 16.1 ± 0.9 7.4 ± 0.4 10.6 ± 0.3 14.7 ± 0.5a
TGF-β1       
 Day 1 19.8 ± 0.4 18.0 ± 0.8 15.5 ± 0.2 9.9 ± 0.2 11.3 ± 0.2 13.3 ± 0.2
 Day 7 18.9 ± 0.4 17.3 ± 0.7 16.9 ± 0.9 7.7 ± 0.5a 9.6 ± 0.4a 14.7 ± 0.5a
IGF-1       
 Day 1 20.0 ± 0.5 16.8 ± 0.6 15.0 ± 0.9 9.1 ± 0.6 11.0 ± 0.2 12.8 ± 0.3
 Day 7 19.8 ± 0.5 18.0 ± 0.7 15.8 ± 0.2 7.1 ± 0.4a 10.0 ± 0.4a 14.6 ± 0.5a
  1. Values represent mean ± standard deviation of all RT-PCR replicates for n = 4 RNA samples. Control values (in bold) at day 1 are used to normalize all data as follows: ∆Ct values of control at day 1 are subtracted from ∆Ct values of control at day 7 or ∆Ct values of each treatment group at both day 1 and day 7 to obtain ∆∆Ct and the fold-difference of relative mRNA levels (2–∆∆Ct) for each target as illustrated in Figure 2. IGF-1, insulin-like growth factor-1; ITG, integrin; TGF, transforming growth factor.
  2. aFold-difference between day 1 and day 7 samples ≥2 and P <0.05, one-factor analysis of variance followed by Tukey’s post-hoc analysis.