Human umbilical cord mesenchymal stromal cells exhibit immature nucleus pulposus cell phenotype in a laminin-rich pseudo-three-dimensional culture system

Table 3 ∆C_tvalues for target genes (target C_t– β2-microglobulin C_t)

Sample	Aggrecan	Collagen II	ITGα6	ITGα3	ITGβ4	CD239
Control
Day 1	21.1 ± 0.7	17.6 ± 0.2	14.6 ± 1.0	8.7 ± 1.0	11.2 ± 0.5	13.3 ± 0.4
Day 7	23.4 ± 0.3	18.3 ± 0.3	16.1 ± 0.9	7.4 ± 0.4	10.6 ± 0.3	14.7 ± 0.5^a
TGF-β1
Day 1	19.8 ± 0.4	18.0 ± 0.8	15.5 ± 0.2	9.9 ± 0.2	11.3 ± 0.2	13.3 ± 0.2
Day 7	18.9 ± 0.4	17.3 ± 0.7	16.9 ± 0.9	7.7 ± 0.5^a	9.6 ± 0.4^a	14.7 ± 0.5^a
IGF-1
Day 1	20.0 ± 0.5	16.8 ± 0.6	15.0 ± 0.9	9.1 ± 0.6	11.0 ± 0.2	12.8 ± 0.3
Day 7	19.8 ± 0.5	18.0 ± 0.7	15.8 ± 0.2	7.1 ± 0.4^a	10.0 ± 0.4^a	14.6 ± 0.5^a

Values represent mean ± standard deviation of all RT-PCR replicates for n = 4 RNA samples. Control values (in bold) at day 1 are used to normalize all data as follows: ∆C_t values of control at day 1 are subtracted from ∆C_t values of control at day 7 or ∆C_t values of each treatment group at both day 1 and day 7 to obtain ∆∆Ct and the fold-difference of relative mRNA levels (2^–∆∆Ct) for each target as illustrated in Figure 2. IGF-1, insulin-like growth factor-1; ITG, integrin; TGF, transforming growth factor.
^aFold-difference between day 1 and day 7 samples ≥2 and P <0.05, one-factor analysis of variance followed by Tukey’s post-hoc analysis.

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