Sample

Aggrecan

Collagen II

ITGα6

ITGα3

ITGβ4

CD239


Control
      
Day 1

21.1 ± 0.7

17.6 ± 0.2

14.6 ± 1.0

8.7 ± 1.0

11.2 ± 0.5

13.3 ± 0.4

Day 7

23.4 ± 0.3

18.3 ± 0.3

16.1 ± 0.9

7.4 ± 0.4

10.6 ± 0.3

14.7 ± 0.5^{a}

TGFβ1
      
Day 1

19.8 ± 0.4

18.0 ± 0.8

15.5 ± 0.2

9.9 ± 0.2

11.3 ± 0.2

13.3 ± 0.2

Day 7

18.9 ± 0.4

17.3 ± 0.7

16.9 ± 0.9

7.7 ± 0.5^{a}

9.6 ± 0.4^{a}

14.7 ± 0.5^{a}

IGF1
      
Day 1

20.0 ± 0.5

16.8 ± 0.6

15.0 ± 0.9

9.1 ± 0.6

11.0 ± 0.2

12.8 ± 0.3

Day 7

19.8 ± 0.5

18.0 ± 0.7

15.8 ± 0.2

7.1 ± 0.4^{a}

10.0 ± 0.4^{a}

14.6 ± 0.5^{a}

 Values represent mean ± standard deviation of all RTPCR replicates for n = 4 RNA samples. Control values (in bold) at day 1 are used to normalize all data as follows: ∆C_{t} values of control at day 1 are subtracted from ∆C_{t} values of control at day 7 or ∆C_{t} values of each treatment group at both day 1 and day 7 to obtain ∆∆Ct and the folddifference of relative mRNA levels (2^{–∆∆Ct}) for each target as illustrated in Figure 2. IGF1, insulinlike growth factor1; ITG, integrin; TGF, transforming growth factor.
 ^{a}Folddifference between day 1 and day 7 samples ≥2 and P <0.05, onefactor analysis of variance followed by Tukey’s posthoc analysis.