Figure 6

Erk1/2 signaling pathway and miR-20b regulate tissue factor expression in trophoblastic and hematopoietic differentiation of human embryonic stem cells. Granulocyte–macrophage (G-M) cells and trophoblasts were treated with both miR-20b inhibitor and Erk1/2-specific inhibitor U0126, simultaneously for 48 hours before harvesting for quantitative real-time polymerase chain reaction to measure the mRNA levels of PU.1, CDX2, and tissue factor (TF). Data reported as the mean ± standard error of the percentage of the mRNA levels of the corresponding gene in cells without treatment. *P <0.05, **P <0.01.