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Table 1 Primer pairs used for reverse transcriptase polymerase chain reaction

From: MicroRNA-20b and ERK1/2 pathway independently regulate the expression of tissue factor in hematopoietic and trophoblastic differentiation of human embryonic stem cells

Gene symbol

Forward primer (5′ to 3′)

Reverse primer (5′ to 3′)

Oct-4

CTTGGGCTACACAGGC

CTCAATACTCGTTCGCTTTC

Nanog

TTTGGAAGCTGCTGGGGAAG

GATGGGAGGAGGGGAGAGGA

CDX2

CCGAACAGGGACTTGTTTAGAG

CTCTGGCTTGGATGTTACACAG

TF

ACGCTCCTGCTCGGCTGGGT

CGTCTGCTTCACATCCTTCA

GAPDH

GGAGCCAAAAGGGTCATC

CCAGTGAGTTTCCCGTTC

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Stem Cell Research & Therapy

ISSN: 1757-6512