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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Human amniotic epithelial cells can differentiate into granulosa cells and restore folliculogenesis in a mouse model of chemotherapy-induced premature ovarian failure

Figure 1

Human amniotic epithelial cells (hAECs) did not express germ-cell-specific genes. (A) Quantitative PCR was used to analyze germ-cell-specific expression in hAECs. CT values were expressed as a percentage of 18S RNA (18S = 100%) and used to calculate mean normalized expression relative to 18S. Results are shown as mean and standard deviation of three experiments. (B) Immunofluorescence analysis of germ-cell-specific genes in hAECs. Note that hAECs expressed OCT4 (red), NANOG (green) and CD117 (green), but did not express DAZL, STELLA or VASA. DAPI staining for nuclei. Scale bars = 50 μm.

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Stem Cell Research & Therapy

ISSN: 1757-6512