Figure 8
From: Ixmyelocel-T, an expanded multicellular therapy, contains a unique population of M2-like macrophages
Ixmyelocel-T alternatively activated macrophages readily phagocytize apoptotic cells. (A) Ixmyelocel-T stained with MerTK. (B-C) Adherent, healthy ixmyelocel-T stained with PKH67 was incubated with PKH26 labeled apoptotic cells. Apoptotic cells were washed away, and healthy ixmyelocel macrophages were analyzed using fluorescence microscopy and flow cytometry. On average 20% percent of ixmyelocel-T CD14+ cells were positive for PKH26+ apoptotic cells after a three hour co-culture apoptotic cells (20 ± 2.9%). (n ≥ 5). Magnification: 60 ×.