Figure 1

Ixmyelocel-T macrophages influx modified cholesterol. (A) Fluorescent microscopy images and (B) flow-cytometry analysis (n ≥ 5) of ixmyelocel-T and THP-1 macrophages loaded with Dil-Ac-LDL. Quantitative real-time PCR gene-expression analysis of scavenger receptors normalized to GAPDH (n ≥ 5). Expression of (C) CD36 and (D) SCARB1 in THP-1 and ixmyelocel-T macrophages before and after lipid loading. Values are presented as mean ± SEM relative to control; ^*P < 0.01 versus THP-1 -Ac–LDL, ^**P < 0.001 versus THP-1 -Ac–LDL. Magnification: 40×. Dil-Ac-LDL, low-density lipoprotein from human plasma, acetylated, Dil complex; PCR, polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; SCARB1, scavenger receptor class B type I; SEM, standard error of the mean; Ac-LDL, acetylated low-density lipoprotein.