Figure 3

Ixmyelocel-T displays enhanced cholesterol-efflux capacity. Quantitative real-time PCR gene-expression analysis of scavenger receptors normalized to GAPDH (n ≥ 5). Expression of (A) ABCA1, (B) ABCG1, (C) ACAT1, and (D) CEH in THP-1 and ixmyelocel-T macrophages before and after lipid loading. (E) Cholesterol efflux of both THP-1 macrophages and ixmyelocel-T to the lipid acceptors ApoA-I and HDL3 (n ≥ 4). Values are presented as mean ± SEM relative to control; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 versus THP-1 -Ac–LDL; ^#P < 0.05, ^##P < 0.01 versus IXT -Ac–LDL. PCR, polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ABC, ATP-binding cassette; ACAT, acyl-CoA:cholesterol acyl-transferase; CEH, cholesterol ester hydrolase; ApoA-I, apolipoprotein A-I; HDL, high-density lipoprotein; SEM, standard error of the mean; Ac-LDL, acetylated low-density lipoprotein.