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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Efficient large-scale generation of functional hepatocytes from mouse embryonic stem cells grown in a rotating bioreactor with exogenous growth factors and hormones

Figure 3

Immunohistochemical and immunocytochemical staining of EBs from the rotating bioreactor. (A) Immunohistochemical staining was performed on sections of day-14 EBs by using anti-ALB, anti-AFP, and anti-CK18 antibodies (red). DAPI was also included for nuclear staining (blue). (B) Immunocytochemical staining for ALB, AFP, and CK18 was performed on the day 14 EB-derived cells cultured on chamber slides for 5 days. Scale bar, 100 μm.

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