Figure 1

Platform set-up diagram. (a) Neural and glial progenitor cells are assembled on a three-dimensional (3D) vascular network formed by endothelial cells, pericytes, and microglia in poly(ethylene glycol) hydrogel to promote the formation of stratified neural epithelium with a vascular network. (b) The neural vascular assembly from (a) will be exposed to a training drug set. The gene expression profiles from the training set will be used to establish a drug toxicity prediction model using a machine learning algorithm. (c) The model established in (b) can be used to predict the toxicity of an unknown chemical.