Figure 12

The protein expressions of oxidative stress and inflammatory biomarkers in both lung and kidney at 72 hours after the CLP procedure with one dose of ADMSC treatment. The protein expressions of oxidized protein (A, F), matrix metalloproteinase (MMP)-9 (B, G), intercellular adhesion molecule (ICAM) (C, H), tumor necrosis factor alpha (TNF-α) (D, I), and nuclear factor (NF)- κB (E, J) in lung and kidney were highest in CLP and lowest in SC, and significantly higher in CLP + H-ADMSC than in CLP + A-ADMSC (all P values <0.005). * versus other groups with different symbols (*, †, ‡, §), P <0.005. DNP, 1–3 dinitrophenylhydrazone; M.W., molecular weight (Note: Right lane and left lane shown on the upper panel represent control oxidized molecular protein standard and protein molecular weight marker, respectively). 1, sham control (SC); 2, cecal ligation and puncture (CLP); 3, CLP + H-ADMSC; 4, CLP + A-ADMSC. All statistical analyses using one-way ANOVA, followed by the Bonferroni multiple comparison post hoc test (n = 6). Symbols (*, †, ‡, §) indicate significance (at 0.05 level). SC, sham control; CLP, cecal ligation and puncture; H-ADMSC, healthy adipose-derived mesenchymal stem cell; A-ADMSC, apoptotic adipose-derived mesenchymal stem cell. ANOVA, analysis of variance.