Figure 1

H & E section of rabbit CACs seeded in a collagen scaffold. Image A is a view of the cell seeded scaffold (Scale bar = 1 mm). Image B is a control scaffold without any CACs seeded (Scale bar = 100 μm). Image C demonstrates the cell seeded scaffold treatment pre-transplantation. The black arrows demonstrate CACs having been seeded on the scaffold for 24 hours. CACs are blue cells, and are predominantly located on the surface of the scaffold. The blue arrow points to the collagen scaffold which is homogeneous in appearance. There are large pores visible in this area of the scaffold. (Scale bar = 100 μm). The surface of the scaffold to which the cells attach is applied directly to the wound bed ensuring the attachment of cells in that area. Image D demonstrates another area of the scaffold which is more porous collagen. This porous appearance of the scaffold was observed at the periphery of the scaffold (orange arrow). A CAC attached to the scaffold is depicted by the black arrow (Scale bar = 100 μm). CACs, circulating angiogenic cells.