Figure 10

Representative images of inflammatory cell infiltrate in wounds. Representative images of hematoxylin and eosin stained histological sections from four treatment groups one week after treatment application are presented. The cells with dark stained nuclei represent neutrophils, macrophages and lymphocytes. Wounds treated with collagen (A) and CACs + collagen (B) reveal increased inflammatory infiltrate in comparison to control wounds (D). Wounds treated with CAC (OPN) + collagen (C) have increased inflammatory cells when compared to control untreated wounds (D) with the density of inflammatory cells appearing less than wounds (A) and (B), suggesting that wounds treated with CAC (OPN) + collagen are progressing more rapidly through the stages of wound healing in comparison to wounds treated with collagen (A) and CACs + collagen (B). This is supported by more vascular structures evident in wounds treated with CAC (OPN) + collagen (C) as compared to other treatment groups. Scale bar = 200 μm. CAC, circulating angiogenic cells; OPN, osteopontin.