Figure 2

Ad-MSCs-SF and D-Ad-MSCs-SF accelerate wound healing in diabetic mice. Diabetic db/db strain mice were used for in vivo experiments. The different SF patches were applied on the wounds and fixed to the skin with 30 μL of hydrogel. A) shows the kinetics of wound closure. At each time the wound area was measured by using a metric ruler that was placed adjacent to the wound as shown in B). Each point at the postoperative day 3, 10, 15 and 20 on the graph are the means ± SD of six mice. Note that on days 3 and 10, mice treated with Ad-MSCs-SF or with D-Ad-MSCs-SF have a significant increment of the wound closure area (*P <0.05, **P <0.01 versus no graft control). C) shows the photographs of the wounds taken at postoperative days 0, 3 and 10. Note that in mice treated with Ad-MSCs-SF and D-Ad-MSCs-SF, the skin wound was significantly reduced compared to either SF or to no graft mice controls. The ameliorative effect was evident even at day 3 after transplantation. In these mice the wounds were almost healed on day 10. Ad-MSCs-SF, silk fibroin patch cellularized with human adipose-derived mesenchymal stromal cells; D-Ad-MSCs-SF, silk fibroin patch after human adipose-derived mesenchymal stromal cells removal (decellularization); SF silk fibroin.