Figure 1

Human cadaver mesenchymal stromal/stem cell isolation, early characterization and expansion. Representative histological staining of native (A) and digested arterial tissue (B) after enzymatic isolation of human cadaver mesenchymal stromal/stem cells (hC-MSCs) (scale bars =10 μm). (C), (D) After harvesting, hC-MSCs collected from three postmortem artery segments show clonogenic activity (scale bars = 50 μm). (E) Numerous poly-nucleated cells (arrow), spindle-shaped cells, dendritic (arrowhead) cells and rounded cells (scale bar = 20 μm). (F) hC-MSC growth kinetics. After 3 weeks of culture, the cells seeded were expanded approximately 20-fold and yielded 250 × 10⁶ cells. (G) ki-67 nuclear immunoreactivity (scale bar = 75 μm). (H) The hC-MSCs at passage 3 became elongated and spindle-shaped with long and thin cytoplasmic projections (scale bar =10 μm).