Figure 3

Human cadaver mesenchymal stromal/stem cell stemness property. Clone-forming potential of (A) a single seeded human cadaver mesenchymal stromal/stem cell (hC-MSC) (arrow) that (B) reached the confluence after 30 days of culture (scale bars = 50 μm). (C) Nonclonogenic single cell with a ring-shaped morphology (scale bar = 10 μm). (D) When plated in nonadhesion conditions, free floating spheres are generated (scale bar = 50 μm). (E) Reverse transcriptase polymerase chain reaction analysis performed on spheroids showed expression of SOX2, OCT-4, c-KIT and KDR. β-Microglobulin was used as the housekeeping gene. The far left lane contains a 100 base pair ladder.