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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Equine bone marrow-derived mesenchymal stromal cells are heterogeneous in MHC class II expression and capable of inciting an immune response in vitro

Figure 2

Trilineage differentiation assay results for MHC class II-negative MSCs, MHC class II-positive MSCs, and dermal fibroblasts (DFs). (A-C) control Oil-Red-O staining of noninduced cell types; bar = 50 μm. (D-F) Oil-Red-O staining after 14 days of adipogenic induction; bar = 50 μm. (G-I) control Alizarin Red staining of noninduced cell types; bar = 200 μm. (J-L) Alizarin Red staining after 14 days of osteogenic induction; bar = 200 μm. (M-O) Alcian blue staining of pellet cultures after 14 days of chondrogenic differentiation; bar = 100 μm. (P-R) Safranin-O/fast green staining of pellet cultures after 14 days of chondrogenic differentiation; bar = 100 μm. Both MHC class II-negative and MHC class II-positive MSCs were able to undergo trilineage differentiation, whereas DFs were not.

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Stem Cell Research & Therapy

ISSN: 1757-6512