Expression level of PLZF in primary mMSC, C3, and C3-PLZF cells. (A) PLZF expression in mMSCs during chondrogenic, adipogenic, and osteogenic differentiation as assessed by RT-qPCR. (B) Expression profile of PLZF in undifferentiated (D0) and differentiated C3 cells into three lineages; adipogenic (Adipo), osteogenic (Osteo), and chondrogenic cells (Chondro). (C) Expression level of PLZF in undifferentiated C3 cells and in a clone of C3 cells with high PLZF expression (C3-PLZF), as quantified by using RT-qPCR. (D) Immunofluorescence analysis of C3 and C3-PLZF by using an anti-PLZF antibody. (E) Expression profile of the master regulators of the chondrogenic, adipogenic, or osteogenic programs, Sox9, PPARγ, or Runx2, respectively, in the C3 or C3-PLZF cells.