Differentiated mDPSC express neuronal and glial markers. A-H) Representative immunohistochemistry images of undifferentiated mDPSC showing strong expression of nestin and ßIII tubulin but low levels of NFM and GFAP. At day 11 of neuronal differentiation mDPSC retained strong nestin and ßIII tubulin expression (A-D). NFM and GFAP expression was markedly increased following neural induction (E-H). I-K) The majority of cells co-expressed ßIII tubulin and GFAP. L-N) Validation of co-expression result using the same ßIII tubulin and GFAP antibodies applied to murine cortical cells, which demonstrates the expected distinction between neurons and glia. O) Quantification of neural IHC. The relative expression of each neural marker before and after differentiation, counted as percent expression per four representative fields of view at 20x magnification. Scale bar = 25 μm. *P <0.05. GFAP, glial fibrillary acidic protein; IHC, immunohistochemistry; mDPSC, murine dental pulp stem cells; NFM, neurofilament-medium chain.