Figure 1
From: Culture bag systems for clinical applications of adult human neural crest-derived stem cells
ITSCs grow three-dimensionally in clinical grade culture bags. (A) Afc-FEP bag containing ITSCs in medium supplemented with human blood plasma. (B) Phase contrast microscopy image of bag-cultured ITSCs revealing characteristic morphology comprising long-shaped cell bodies. (C) Confocal laser scanning microscopy analyses (Z-sectioning) followed by three-dimensional-reconstruction showed three-dimensional-growth of bag-cultured ITSCs previously transfected with GFP. FEP, fluorinated ethylene propylene; ITSCs, inferior turbinate stem cells.