Figure 4

Quantitative real-time PCR of four hCBiPSC lines. Relative expression levels of pluripotency (A), neural progenitor (B) and neuronal and dopaminergic markers (C) in four hCBiPSC clones (K1, K2, K106, K120). (A) The expression of the pluripotency markers Oct4 and Lin28 is significantly decreased in most cell lines during expansion whereas the neural stem cell markers Sox1 and Pax6 (B) are markedly upregulated in all hCBiPSC clones after differentiation with DM/SB for four days. (C) After 40 days of differentiation in vitro the relative expression of the neuronal markers Tuj1 and MAP2 is significantly enhanced in almost all clones. Furthermore, the expression levels of tyrosine hydroxylase (TH) and the dopamine transporter (DAT) which are markers for dopaminergic neurons, as well as the mesencephalic marker engrailed 1 (En1) are significantly elevated in most cell lines. Results are reported as means ± SEM. P-values (*P <0.05, **P <0.01, ***P <0.001) were calculated using an unpaired t-test. Abbreviations: EXP, expanded cells; DIFF, differentiated cells. DM, dorsomorphin; hCBiPSC, human cord blood induced pluripotent stem cells; SEM, standard error of the mean.