Figure 7
Differentiated hCBiPSCs showed KCl- and neurotransmitter-mediated Ca2+signaling. (A) hCBiPSC-K2 were loaded with Fura-2 after four weeks of differentiation in vitro. (B) Transient cytosolic Ca2+ changes of three representative cells induced by bath application of acetylcholine (ACh, 100 μM), GABA (100 μM), glutamate (50 μM) and KCl (50 mM). Intracellular calcium concentrations are presented as ratios of the fluorescence signals obtained at 340 and 380 nm (F340/F380). (C) Fractions of cells responding to ACh (25%), GABA (62%), glutamate (22%) and KCl (68%) obtained from experiments as shown in (B). (D) Summary of cytosolic Ca2+ response amplitudes given as ratio F340/F380 (left) and intracellular calcium concentration ([Ca2+]i, right) both normalized to the basal calcium level of the cells. Basal intracellular Ca2+ level was 100 ± 8 nM (n = 57 cells). Note, GABA induced depolarizing effects in most investigated cells. All data are given as mean ± SEM. hCBiPSC, human cord blood induced pluripotent stem cells; SEM, standard error of the mean.