Figure 2

Differentiation markers and multipotent differentiation capacity in PSFCs. (a) Differentiation markers of PSFCs. Negative control was incubated with only secondary antibodies (a-c). Nestin (d), Musashi-1 (f), osteocalcin (OCN)(g), αSMA (h), Tuj-1 (i) and MAP-2 (j). Scale: 100 μm. (b) Multipotent differentiation capacity of PSFCs. PSFCs were differentiated under specific culture conditions suitable for differentiation into each cell type. Identification of differentiation: Alizarin red staining for mineralized cells; Oil red O staining for adipocytes; αSMA immunostaining for myocytes. Cont, before differentiation; Diff,: after differentiation. Scale: 100 μm.