Figure 6

SVF CD14⁺cells incorporate into new vessels. Fourteen days after implantation, Matrigel implants containing SVF CD14⁺ cells were evaluated for the expression of CD31, CD45, and e-NOS. (A) Representative images for CD31 staining. Arrows indicate the presence of CD31⁺ endothelial cells in the vessels formed. (B) Human CD45 immunostaining. Note that CD45⁺ cells are located surrounding blood vessels (left) or adjacent to the endothelium (right). (C) Human e-NOS immunostaining. Arrows indicate positive staining of some blood vessels for e-NOS. Scale bars: A, 50 μm; B, 50 μm (left), 30 μm (right); C, 50 μm.