Figure 7

Matrigel implants contain blood vessels comprising human and mouse endothelial cells. Consecutive sections of Matrigel implants with SVF CD14⁺ cells were double-immunostained with antibodies against mCD31 (green) and αSMA (red) (A), or Ulex europaeus agglutinin 1 (UEA-1; green) and αSMA (red) (B). The images on the top of the panels A and B show merged images and correspond to the same microscopic field at different magnifications: left, low magnification; middle, medium magnification; right, higher magnification. Images on the bottom (A, B) show green and red fluorescence and correspond to the merged image at the highest magnification. Images in panel B correspond to a consecutive section. The insets in A and B (upper left) show the field at low magnification and asterisks illustrate benchmarks. Note that the green staining associated with murine (A) or human (B) endothelial cells in the adjacent sections is near identical. Double labeling shows a close assembly of αSMA-positive cells to the blood vessels. White arrows indicate αSMA-positive cells lining vascular-like structures which do not express murine (A) or human (B) endothelial cells. Scale bars in A, B (upper left): 200 μm, upper in the middle: 100 μm; upper right and bottom: 50 μm.