The isolation and characterization of hGMSCs. (A) The isolation protocol/timeline of hGMSCs is illustrated. (B) The colony of isolated hGMSCs was stained with crystal violet. (C) The mesenchymal stem cell surface markers, which are CD90, CD73 and CD105, were highly expressed in hGMSCs, but STRO-1 was expressed at a low level. (D) The telomerase activity of hGMSCs was significantly higher than that of gingival fibroblasts. (E) Multi-potency in the differentiation of these isolated hGMSCs was successfully induced and characterized, including the osteogenic, adipogenic and neural differentiations. hGMSCs, human gingiva-derived mesenchymal stem cells.