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Table 1 Cumulative population doubling times for mesenchymal stromal cells grown in α-MEM supplemented with 10% FBS

From: Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton’s jelly

Time (hours)

BM-MSC

AT-MSC

WJ-MSC

96

79.5 ± 16.1

59.8 ± 3.3

28.4 ± 3.6

144

79.7 ± 2.1

51.5 ± 3.4

34.0 ± 1.8

192

88.4 ± 8.1

63.5 ± 6.6

38.4 ± 1.7

  1. Cell pools were plated in 24-well plates at a concentration of 6,000 cells/mL and were trypsinized and counted after 96, 144 and 192 hours.
  2. FBS: fetal bovine serum, α-MEM: alpha-Minimum Essential Medium, AT-MSC: adipose tissue-derived mesenchymal stromal cells, BM-MSC: bone marrow-derived mesenchymal stromal cells; WJ-MSC: Wharton’s Jelly-derived mesenchymal stromal cells (WJ-MSC).
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Stem Cell Research & Therapy

ISSN: 1757-6512