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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: RETRACTED ARTICLE: Umbilical cord tissue-derived mesenchymal stem cells induce apoptosis in PC-3 prostate cancer cells through activation of JNK and downregulation of PI3K/AKT signaling

Figure 1

Characterization of mesenchymal stem cells. (A) Beta-galactosidase staining was used to check SA-β-gal activity in early passages (0, 1, 3, and 5) of hUCMSCs. Cell morphology was observed by phase-contrast microscopy. Scale bar, 50 μm. (B) Growth kinetics of the hUCMSCs according to the passages of hUCMSCs. (C) Characterization of isolated hUCMSCs was performed by identification of MSC markers, OCT4 (green) and NANOG (red). Nuclei were stained with DAPI (blue). Scale bar, 50 μm. (D) Characterization of isolated hUCMSCs cultured in adipogenic and osteogenic differentiation media for 14 days was performed by identification of the adipogenic and osteogenic differentiation assays, as described in Materials and Methods. The cells were stained with Oil Red-O and Alizarin Red dye staining, respectively. Scale bar, 100 μm.

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Stem Cell Research & Therapy

ISSN: 1757-6512