Immortalization of human adipose-derived stromal cells: production of cell lines with high growth rate, mesenchymal marker expression and capability to secrete high levels of angiogenic factors

Table 3 Relative expression percentages of surface markers analyzed by flow cytometry

	hASC-M	hASC-T	hASC-TS	hASC-TE
CD90	81.0 ± 3.4	70.0 ± 17.0	96.3 ± 1.6	77.0 ± 21.0
CD73	94.0 ± 2.4	96.0 ± 2.1	85.0 ± 2.3	92.5 ± 3.6
CD105	83.0 ± 5.1	81.0 ± 0.3	48.1 ± 2.0	37.0 ± 16.0
CD34	1.5 ± 0.1	1.2 ± 0.5	0.6 ± 0.1	0.9 ± 0.3
CD45	0.8 ± 0.3	1.2 ± 0.1	1.0 ± 0.2	0.5 ± 0.1
HLA-I	96.3 ± 2.1	80.0 ± 8.0	98.0 ± 0.4	92.0 ± 6.0
HLA-II	0.6 ± 0.2	0.8 ± 0.4	1.6 ± 0.6	0.9 ± 0.3
CD44	97.0 ± 0.4	94.0 ± 3.0	98.7 ± 0.2	99.5 ± 0.3
CD106	12.0 ± 6.0	30.5 ± 12.0	12.0 ± 0.2	46.8 ± 19.8
CD146	4.8 ± 1.0	4.1 ± 0.4	26.0 ± 6.0	60.0 ± 14.0
SSEA-4	23.0 ± 2.8	29.0 ± 3.0	16.0 ± 6.0	30.0 ± 12.0

Phenotypic panel of markers analyzed by flow cytometry on hASC-M, hASC-T, hASC-TS and hASC-TE cells. Relative expression percentages of each antigen are reported. Values are the mean ± S.E. of five independent experiments.

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