Skip to main content
Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Lipopolysaccharide differentially affects the osteogenic differentiation of periodontal ligament stem cells and bone marrow mesenchymal stem cells through Toll-like receptor 4 mediated nuclear factor κB pathway

Figure 2

LPS impairs the osteogenic potential of PDLSCs and activates the NF-κB pathway in PDLSCs and BMMSCs. (A) PDLSCs and BMMSCs treated with or without LPS were cultured in osteogenic medium and osteogenic differentiation was determined by Alizarin red S staining after 28 days. Alizarin red was then extracted and measured for light absorbance at 620 nm. (B) Gene expression of Runx2 in PDLSCs and BMMSCs with or without LPS treatment was measured by qRT-PCR after osteogenic induction for 14 days. (C) Western blot analysis showed the protein expression of phospho-IκBα, IκBα, phospho-NFκBp65 and NFκBp65, and β-actin was used as the internal control. (D, E) Relative intensity of the tested protein was quantitatively analyzed using Adobe Photoshop CS2 software. Data represent the means ± SD. * P <0.05 (n = 3). BMMSCs, bone marrow mesenchymal stem cells; LPS, lipopolysaccharide; NF-κB, nuclear factor κB; PDLSCs, periodontal ligament stem cells; SD, standard deviation.

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512