Figure 2

Effect of exosomes on hepatocyte proliferation after CCl ₄ -induced injury in mice. (A) Expression of cyclin E, HGF, pMet, NF-κB p65, cyclin D1, and phosphorylated STAT3 was determined by immunoblotting after 24 hours of CCl₄ administration with or without exosomes (n = 6 per group (three per group were shown); *P < 0.05 versus CCl₄ control). The relative densities of all the proteins bands were normalized to actin in the same samples. Livers were processed for immunohistochemistry on PCNA to quantify hepatocyte proliferation after 24 hours of (B) CCl₄ administration and (C) CCl₄ with exosomes treatment. Original magnification × 200; scale bar, 100 μm.