Table 1 Sequences of primers used in real-time PCR reaction
Gene | Forward primer (5ʹ- > 3ʹ) | Reverse primer (5ʹ- > 3ʹ) |
|---|---|---|
In vitro hepatocytes (TAMH cells)/In vivo liver (mouse) | ||
TNF-α | ATG AGC ACA GAA AGC ATG ATC | TAC AGG CTT GTA ACT CGA ATT |
IL-6 | AGT TGC CTT CTT GGG ACT GA | TCC ACG ATT TCC CAG AGA AC |
iNOS | CAC CTT GGA GTT CAC CCA GT | ACC ACT CGT ACT TGG GAT GC |
COX-2 | CTC CCT GAA GCC GTA CAC AT | GCT CGG CTT CCAA GTA TTG AG |
MIP-2 | AAG TTT GCC TTG ACC CTG AA | AGG CAC ATC AGG TAC GAT CC |
HO-1 | CAG GTG ATG CTG ACA GAG GA | ATG GCA TAA ATT CCC ACT GC |
Gpx4 | CCG GCT ACA ACG TCA AGT TT | CGG CAG GTC CTT CTC TAT CA |
Gsr | ACC ACG AGG AAG ACG AAA TG | GGT GAC CAG CTC CTC TGA AG |
MnSOD | GGC CAA GGG AGA TGT TAC AA | CCT TGG ACT CCC ACA GAC AT |
GAPDH | GGC ATT GCT CTC AAT GGAC AA | CCG AGGT TGG GAT AGG GCC |