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Table 3 Percentage expression of MSC-positive and negative markers, by cells cultured in MesenCult XFSM medium and in DMEM KO + 10% FBS medium, at passages 3 and 5

From: Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study

KO + FBS P3 P5 XF,SFM P3 P5
CD markers Average ± SD Average ± SD CD Markers Average ± SD Average ± SD
CD 73 93.4 ± 1.7 85.9 ± 15.7 CD 73 94.3 ± 4.09 96.5 ± 0.68
CD 34 5.1 ± 2.3 4.2 ± 3.2 CD 34 3.3 ± 2.5 3.7 ± 1.04
CD 44 92.6 ± 1.4 85.9 ± 15.8 CD 44 95 ± 3 96.9 ± 2.7
CD 166 81.6 ± 8.2 84.7 ± 0.4 CD 166 82.2 ± 11.09 82 ± 2.7
CD 90 98.5 ± 0.6 95.9 ± 1.9 CD 90 98.2 ± 0.6 99.1 ± 0.7
CD 200 52 ± 7.5 46.2 ± 8.8 CD 200 13.5 ± 12.3 4.5 ± 5.6
CD 271 0.6 ± 0.2 0.33 ± 0.2 CD 271 2.2 ± 1.8 0.75 ± 0.2
CD 105 85.5 ± 5.5 71.5 ± 17.0 CD 105 97.7 ± 1.9 81.1 ± 7.2
HLA DR 0.7 ± 0.7 3 ± 2.1 HLA DR 2.9 ± 2.5 0.76 ± 0.73
CD 45 0.4 ± 0.5 4.4 ± 3.1 CD 45 0.23 ± 0.2 0.8 ± 0.7
CD 80 2.7 ± 0.4 0.13 ± 0.05 CD 80 2.6 ± 2.9 0.9 ± 0.8
HLA ABC 73 ± 16.9 41.4 ± 8.6 HLA ABC 81.06 ± 1.8 72.3 ± 23.6
  1. MSC, mesenchymal stem cell; P, passage; SD, standard deviation.