Table 3 Percentage expression of MSC-positive and negative markers, by cells cultured in MesenCult XFSM medium and in DMEM KO + 10% FBS medium, at passages 3 and 5
KO + FBS | P3 | P5 | XF,SFM | P3 | P5 |
|---|---|---|---|---|---|
CD markers | Average ± SD | Average ± SD | CD Markers | Average ± SD | Average ± SD |
CD 73 | 93.4 ± 1.7 | 85.9 ± 15.7 | CD 73 | 94.3 ± 4.09 | 96.5 ± 0.68 |
CD 34 | 5.1 ± 2.3 | 4.2 ± 3.2 | CD 34 | 3.3 ± 2.5 | 3.7 ± 1.04 |
CD 44 | 92.6 ± 1.4 | 85.9 ± 15.8 | CD 44 | 95 ± 3 | 96.9 ± 2.7 |
CD 166 | 81.6 ± 8.2 | 84.7 ± 0.4 | CD 166 | 82.2 ± 11.09 | 82 ± 2.7 |
CD 90 | 98.5 ± 0.6 | 95.9 ± 1.9 | CD 90 | 98.2 ± 0.6 | 99.1 ± 0.7 |
CD 200 | 52 ± 7.5 | 46.2 ± 8.8 | CD 200 | 13.5 ± 12.3 | 4.5 ± 5.6 |
CD 271 | 0.6 ± 0.2 | 0.33 ± 0.2 | CD 271 | 2.2 ± 1.8 | 0.75 ± 0.2 |
CD 105 | 85.5 ± 5.5 | 71.5 ± 17.0 | CD 105 | 97.7 ± 1.9 | 81.1 ± 7.2 |
HLA DR | 0.7 ± 0.7 | 3 ± 2.1 | HLA DR | 2.9 ± 2.5 | 0.76 ± 0.73 |
CD 45 | 0.4 ± 0.5 | 4.4 ± 3.1 | CD 45 | 0.23 ± 0.2 | 0.8 ± 0.7 |
CD 80 | 2.7 ± 0.4 | 0.13 ± 0.05 | CD 80 | 2.6 ± 2.9 | 0.9 ± 0.8 |
HLA ABC | 73 ± 16.9 | 41.4 ± 8.6 | HLA ABC | 81.06 ± 1.8 | 72.3 ± 23.6 |