Figure 5

MiR-671-5p targeting on soluble tumor necrosis factor receptor type II and interlukin-1 receptor agonist in orbital fat-derived stem cells. (A) Co-culture with activated macrophages significantly reduced miR-671-5p in orbital fat-derived stem cells (OFSCs), and transfection of miR-671-5p inhibitor successfully inhibited miR-671-5p expression in OFSCs. Analysis of variance with Tukey’s post hoc test, different characteristics represented a different level of significance at 95% confidence interval, n = 3. (B) Transfection of miR-671-5p selectively upregulated interlukin-1 receptor agonist ( IL-1RA) and soluble tumor necrosis factor receptor type II (sTNFRII) expression in OFSCs. t test, *P < 0.05, n = 3. miR-671-5p showed a strong binding affinity to sTNF RII (C) and IL-1RA (D) by a low minimal free energy and a high miRanda alignment score. (E) Inhibition of miR-671-5p enhanced the protein expressions of sTNFRII and IL-1RA in OFSCs. IDO, indoleamine 2,3 dioxygenase; IL, interleukin.