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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Influence of insulin-like growth factor I overexpression via recombinant adeno-associated vector gene transfer upon the biological activities and differentiation potential of human bone marrow-derived mesenchymal stem cells

Figure 1

Recombinant adeno-associated virus-mediated IGF-I gene transfer in undifferentiated monolayer cultures of human bone marrow-derived mesenchymal stem cells. Cells were transduced with rAAV-hIGF-I or rAAV-lacZ (20 μl each vector) as described in Methods and histologically processed after 21 days to monitor (A) transgene (insulin-like growth factor I) expression by immunocytochemical analysis and enzyme-linked immunosorbent assay, (B) levels of cell proliferation by immunocytochemical detection of bromodeoxyuridine (BrdU) incorporation and histomorphometry, by WST-1 assay, and by detection of the DNA contents, and (C) levels of proteoglycan synthesis (magnification × 4). *Statistically significant compared with rAAV-lacZ. hIGF-I, human insulin-like growth factor I; OD, optical density; rAAV, recombinant adeno-associated virus.

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Stem Cell Research & Therapy

ISSN: 1757-6512