Figure 3

Effect of inflammatory cytokines on adipogenesis of mouse mesenchymal stem cells (MSCs) in vitro . Adipogenesis was suppressed by tumor necrosis factor-alpha (TNFα) and interleukin-1-beta (IL-1β) in a dose-dependent manner in (A) BALB/c MSCs compared with positive control cultures differentiated in the absence of pro-inflammatory cytokines. Negative controls represent MSCs cultured in complete expansion medium only. A similar trend was seen in (B) FVB MSCs but did not reach statistical significance. The effect of pro-inflammatory cytokines on BALB/c adipogenesis was also demonstrated by Oil Red O staining (C). A representative image of Oil Red O staining of FVB cells cultured in adipogenic medium is shown (D). Data shown are mean absorbance of dissolved Oil Red O ± standard error of the mean of triplicate experiments. *P <0.05, **P <0.001 (two-way analysis of variance).