Figure 6

Pre-stimulation (PS) with pro-inflammatory cytokines increased migration of murine mesenchymal stem cells (MSCs) toward fetal bovine serum (FBS) in vitro independently of the nuclear factor-kappa-B (NF-κB) pathway. In BALB/c (A) and FVB (B) MSCs, a significant increase in migration toward 10% FBS was demonstrated compared with serum-free medium controls. PS of BALB/c and FVB MSCs with 50 ng/mL of tumor necrosis factor-alpha (TNFα) or interleukin-1-beta (IL-1β) for 24 hours increased migration of MSCs toward FBS medium compared with non-PS MSCs. Transduction of MSCs with AdsrIκB did not reverse the effect of pre-stimulation (C). Similarly, addition of 25 μM curcumin did not reverse the effect of pre-stimulation with TNFα (D). Data shown are the mean ± standard error of the mean of triplicate experiments. *P <0.05, **P <0.001 (two-way analysis of variance).