TNFα and IL-1β influence the differentiation and migration of murine MSCs independently of the NF-κB pathway

Table 1 Absolute decrease in calcium per well of mesenchymal stem cells undergoing osteogenesis in the presence of pro-inflammatory cytokines

	Decrease in absolute calcium per well, mean μg (SD)		P value
	BALB/c	FVB	P value
10 ng/mL TNFα	10.72 (0.55)	16.71 (1.47)	0.03^a
1 ng/mL TNFα	10.6 (0.41)	5.4 (2.61)	0.09
0.1 ng/mL TNFα	2.35 (0.54)	4.25 (3.45)	0.36
10 ng/mL IL-1β	11.64 (0.03)	16.52 (4.97)	0.23
1 ng/mL IL-1β	11.45 (0.20)	17.88 (1.87)	0.03^a
0.1 ng/mL IL-1β	5.57 (4.36)	8.16 (4.44)	0.65

The absolute decrease in calcium per well of FVB and BALB/c cells stimulated to undergo osteogenesis in the presence of pro-inflammatory cytokines. A decrease in calcium production was demonstrated in all wells with response by FVB cells statistically greater than that for BALB/c preparations in two culture conditions. ^aP <0.05. IL-1β, interleukin-1-beta; SD, standard deviation; TNFα, tumor necrosis factor-alpha.

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