Figure 7

Colocalization of OCT-4 and PDX-1. Colocalization of OCT-4 and PDX-1 in four different cells (i, iv, vii and x) on smears prepared from pancreatic tissue collected on day 5 post pancreatectomy. Both OCT-4 (red) (i, iv, vii, x) and PDX-1 (green) (ii, v, viii, xi) colocalize in the nuclei and thus yellow merged images are obtained (iii, vi, ix, xii). It is interesting to note that although PDX-1 is restricted to the nucleus, in places OCT-4 also shows cytoplasmic localization (vii and ix). Results confirm the presence of PDX-1-positive and OCT-4-positive pancreatic progenitors on day 5 after pancreatectomy. PDX-1-positive cells showing the presence of nuclear-to-cytoplasmic OCT-4 shows that they are derived from the nuclear OCT-4-positive very small embryonic-like stem cells. Scale bar represents 20 μm.